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Innovation Chimie Carnot NORMANDIE_UNIVERSITE


technical platformsProteomique Pissaro

Proteomique Pissaro

The PISSARO proteomics platform was created in 2003 to provide expertise and effective technological means recognized to the academic and industrial scientific community in the field of separation, identification and quantification of proteins and peptides.

The acquisitions available on the PISSARO platform were obtained thanks to funding (Region, ERDF, MESR, IBiSA, ANR, charitable associations), leading to an instrumental park which represents more than 3 million euros of investment.

A qualified staff is present in the various departments of the platform. Thus 17 members (researchers, engineers) participate in the activity of the platform and their mission is oriented towards the operation, development activities and maintenance of specific fields of instrumentation.

An ISO 9001 quality certification has been obtained for nearly 10 years. Its objective is to implement quality through a comprehensive and structured procedure. This approach extends to all platform activities and encompasses all aspects of excellence: user and customer satisfaction, constant quality improvement, cost and time control, and innovation process. The platform has received a new certification for the instrument / technology platform: NFX-50-900 and is now ISO9001 / V2015 certified.



















 

The platform is widely open to national and foreign research laboratories, whether academic or private. The PISSARO platform has been involved in numerous ANR projects and in many international programs. In addition, the platform has built a network of international collaborations and has developed an important partnership with private companies (BMS, Pfizer, MSD, Stago, Corrodys, Proteomic Solutions, GreenTech, Lactalis, Stallergenes, BioSIMS and Medicago). A privileged partnership has been established with Agilent Technologies and the PISSARO platform is today "Associated Lab" with this company, leader in the field of scientific instrumentation.

The platform is also very involved in higher and continuing training. Students and PhD students have access to the PISSARO platform to carry out their research projects (French and foreign students). In the framework of various partnerships, trainings or transfer of know-how sessions were also organized.

Scientific strategy

The main objective of the PISSARO platform is to offer the scientific community an infrastructure dedicated to the study of proteins in a wide range of analytical situations and to promote innovation in order to broaden the repertoire of strategies proposed.
Although the platform is widely open to protein analysis in all areas, specific developments around targeted applications have been identified as priorities:
  •     Translational research with the identification of biomarkers for diagnostic, prognostic and theranostic purposes, particularly in the context of autoimmune diseases,
  •     The characterization of glycoproteins and in particular glycosylated therapeutic proteins,
  •     The search for protein targets involved in the mechanisms of adhesion and bacterial resistance,
  •     The development of expertise and know-how in the field of post-translational modifications,
  •     The research and quantification of endogenous peptides in complex matrices such as biological tissues or fluids.
     

















These projects are carried out with our various partners and some of them are cited below as illustrative examples through the recent publications (Peixoto et al, JACS 2014, Ciapuccini et al, Eur J Endocrinol 2014, Maury et al, Anal Chem 2014, Pournaras et al, AAC 2014, Salzano et al, PNAS 2014, Obry et al, Theranostics 2015, Breton et al, Cell Metab 2015, Trouillard et al., Anal Chem, 2015, Ouidir et al., Proteomics 2015, Mullen Et al, Sci Rep 20105, Young et al, Autophagy 2015, Arfi et al, PNAS 2016, Mezni et al, Molec Neurobiol 2016, Doctor et al, J Cereb Blood Flow Metab 2016, Thakur et al, Cell Death DIff 2016, Clamens Et al., Sci Rep 2017; Kentache et al., MCP 2017).

Departments and instrumentation

The platform is composed of 3 departments (electrophoresis, mass spectrometry and chromatography, micro-sequencing) and has just moved into a new building (CURIB) with dedicated premises and occupies more than 750 m2 at the University of Rouen On the site of Mont Saint Aignan (France).

 
The main equipment acquired in recent years is presented below:

Mass spectrometry and chromatography


  •     Easy-nLC / LTQ-Orbitrap Elite (Thermo Scientific),
  •     UPLC 1290 / QQQ 6490 with iFunnel Technology (Agilent),
  •     Ultimate 3000 / Q-Exactive (Thermo Scientific),
  •     NanoLC / chip / QTOF 6520 (Agilent),
  •     NanoLC / chip / Ion Trap 6340 (Agilent),
  •     MALDI-TOFTOF Ultraflex (Bruker),
  •     Ultimate 3000-probot (Dionex),
  •     ProteomeLab ™ PF 2D (Beckman).





















Microsequencing of proteins

  •     Procise 492 (Applied Biosystems),
  •     Procise 494 (Applied Biosystems),
  •     Microblotter (Applied Biosytems).

Electrophoresis

  •     IPGPhor (Pharmacia),
  •     Multiphore II (Pharmacia),
  •     IEFCell (BioRad),
  •     PROTEAN II XL (BioRad),
  •     Dodeca Cell Criterion (BioRad),
  •     Typhoon ™ FLA 9500 Biomolecular Imager (GE Healthcare),
  •     OffGel system (Agilent).

Dedicated PLCs
  •     Multiprobe II (Perkin Elmer),
  •     Biomek3000 (Beckman),
  •     JANUS (Perkin Elmer),
  •     Ettan ™ Spot Picker (Amersham),
  •     Ettan ™ Digester (Amersham),

Dedicated software
  •     SameSpots (TotalLab),
  •     Spectrum Mill (Agilent),
  •     Progenesis QI (Waters),
  •     Proteome Discoverer (Thermo Scientific),
  •     Mascot (Matrix Sciences),
  •     Peaks Studio (Bioinformatics Solutions),
  •     Mass Profiler (Agilent),
  •     Ingenuity Pathway Analysis (QIAGEN Bioinformatics).


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